Micro and Macro Anatomical Studies of Spleen at Different Stages of Development in Pati Duck (Anas platyrhynchos domesticus) of Assam.

Abstract:

The study on the micro and macro anatomy of the spleen of Pati duck of Assam is of great value regarding the diseases control regime. The study aimed to evaluate the gross and histomorphology examination of the spleen of the immune system. For this study, thirty-six numbers of Pati ducks were utilized. The duck was divided into six groups depending on its age viz., day old, 15 days, 4 weeks, 8 weeks, 16 weeks, and 24 weeks. For a collection of the spleen, a ventro median incision was given in the abdominal region, the spleen was exposed, its topography was studied in situ and the organ was separated from the body. The research was carried out for two years in the Department of Anatomy & Histology, College of Veterinary Science, Assam Agricultural University, Khanapra, Guwahati, Assam. The pieces of spleen were collected immediately after slaughter and fixed in a 10% neutral buffered formalin solution and were processed as per the standard technique of procedure (Luna, 1968). The paraffin blocks were sectioned in Shandon Finesse microtome at 5µm thickness and the sections were stained with Mayer’s Haematoxylin and Eosin staining technique for Cellular details Van Gieson’s method for collagen fiber, Gomori’s method for reticular fibre, Hart’ method for elastic fibre and Bielchowsky’s method for axis cylinder and dendrites as per the method of Luna (1968). The spleen of Pati duck of Assam was located close to the right side of the junction between the proventriculus and gizzard adjacent to the liver in close association with the gall bladder. It was reddish brown. The spleen of Pati duck of Assam is encapsulated by a connective tissue capsule from which connective tissue fibres penetrated the parenchyma as trabeculae. Spleen parenchyma is comprised of red pulp and white pulp. It was observed that fine reticular fibres formed the meshwork which supported the splenic parenchyma.